lacZY gene fusion cassettes with KanRresistance
نویسندگان
چکیده
منابع مشابه
Functional gene cassettes in development.
It has been noted in recent years that groups ofgenes are often used at multiple stages and multiple places of development. Comparison of the functions of each group of genes in different developmental context gave rise to the notion of "functional gene cassettes." These groups of genes can often be viewed as sets of tools; each group is suited to carry out a certain operation and may serve sim...
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Integrons were detected in 59 of 120 (49%) urinary isolates of Enterobacteriaceae by PCR using degenerate primers targeted to conserved regions of class 1, 2, and 3 integrase genes. PCR sequencing analysis of the cassette arrays revealed a predominance of cassettes that confer resistance to the aminoglycosides and trimethoprim.
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OBJECTIVES The transposon Tn1331 possesses a region including three antibiotic resistance genes with the structure aac(6')-Ib-attC-aadA1-attI1*-bla(OXA-9)-attC, which potentially includes four gene cassettes. Experimental data on the mobility of fusion cassettes as well as those on mobility of cassettes in a genetic environment such as Tn1331, which lacks an integrase gene, are limited. Therefo...
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Salmonella enterica is a versatile live carrier for the presentation of recombinant vaccine antigens. Fusion proteins of a type III secretion system effector and heterologous vaccine antigens can be translocated by live attenuated Salmonella strains and mediate protective immunity against infections. Here we investigated the use expression cassettes for translocated fusion protein consisting of...
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I ntegrons, which are natural genetic engineering platforms, can incorporate open reading frames and convert them to functional genes by ensuring correct expression. Although such integrons play a major role in multidrug resistance phenomena among gram-negative species, similar platforms occur in numerous bacterial species, where they can play other roles. Meanwhile, we have evidence suggesting...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.8.3587